pedro carvalho lipid

Universitat de Barcelona P. Carvalho, V. Teixeira, and L. Johnsen designed the experiments and analyzed most of the data. https://doi.org/10.7554/eLife.07253 Organisms and cells face a myriad of environmental changes with periods of nutrient surplus and shortage. https://doi.org/10.1242/jcs.076836 Vitor Teixeira Distinct ubiquitin-ligase complexes define convergent pathways for the degradation of ER proteinsIdentification of seipin-linked factors that act as determinants of a lipid droplet subpopulationYMR313c/TGL3 encodes a novel triacylglycerol lipase located in lipid particles of Saccharomyces cerevisiaeNext, we tested the contribution of each Ldo isoform to the recruitment of Pdr16 to LDs. 0000-0002-2620-4007 https://doi.org/10.1016/j.tcb.2016.02.007Changes in lipid composition during nutrient deprivation drive vacuolar lipid phase segregation into liquid-ordered (Lo) and disordered domains.
https://doi.org/10.1073/pnas.0704154104Large-scale purification of TAP-tagged proteins for mass spectrometry analysis was performed as described previously ( https://doi.org/10.1016/j.devcel.2015.04.015Seipin regulates ER-lipid droplet contacts and cargo deliveryLipid droplet size directs lipolysis and lipophagy catabolism in hepatocytes https://doi.org/10.1016/j.celrep.2016.10.037Cell and Developmental Biology Programme, Centre for Genomic Regulation, Barcelona, SpainThe lipodystrophy protein seipin is found at endoplasmic reticulum lipid droplet junctions and is important for droplet morphologyRetrotranslocation of a misfolded luminal ER protein by the ubiquitin-ligase Hrd1pAdditional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae In the summer of 2016, I was appointed the EP Abraham Professor of Cell Biology at the University of Oxford and established my lab at the Sir William Dunn School of Pathology.Teixeira V, Johnsen L, Martínez-Montañés F, Grippa A, Buxó L, Idrissi FZ, Ejsing CS, Carvalho P., 'Regulation of lipid droplets by metabolically controlled Ldo isoforms'. https://doi.org/10.1016/j.bbalip.2011.07.015SEIPIN Regulates Lipid Droplet Expansion and Adipocyte Development by Modulating the Activity of Glycerol-3-phosphate AcyltransferaseSeipin performs dissectible functions in promoting lipid droplet biogenesis and regulating droplet morphologyThe authors declare no competing financial interests.Targeting Fat: Mechanisms of Protein Localization to Lipid Droplets Loges Krshnan. https://doi.org/10.1091/mbc.E15-03-0173 4 ), a feature that sets them apart from canonical MCSs. “la Caixa” Foundation Fld1p, a functional homologue of human seipin, regulates the size of lipid droplets in yeastThis article is available under a Creative Commons License (Attribution 4.0 International, as described at https://doi.org/10.1146/annurev-biochem-061009-102430A Phosphatidylinositol Transfer Protein Integrates Phosphoinositide Signaling with Lipid Droplet Metabolism to Regulate a Developmental Program of Nutrient Stress-Induced Membrane BiogenesisTo study the impact of deregulating Ldo45 levels, we replaced Protein areas were normalized intra- and intersamples by the median of the Log area. Ejsing performed and analyzed lipidomics experiements.
https://doi.org/10.15252/embj.201695170 Grids were imaged using a Tecnai 12 transmission electron microscope (FEI) operated at 120 kV with a OneView digital camera (Gatan).Transition from exponential to stationary phases is triggered by nutrient deprivation and involves dramatic rewiring of cellular metabolism, particularly at the level of lipid biosynthesis and turnover (LDs consist of neutral lipids in their core surrounded by a phospholipid monolayer and specific proteins essential in regulating their growth and consumption (L. Johnsen was supported by a La Caixa Foundation graduate fellowship.

TCA-precipitated proteins were resuspended in 6 M urea and 200 mM ammonium bicarbonate before reduction (10 mM DTT) and alkylation (20 mM iodoacetamide). Search for other works by this author on:

EM samples were prepared at the Science and Technology Centres of the University of Barcelona and imaged at the Sir William Dunn School of Pathology EM Facility. In contrast, Ldo16 does not affect LD proteome and functions primarily upon nutrient depletion, facilitating LD consumption by lipophagy. In particular, I focus on how organelle function is maintained through quality control processes, such as protein degradation. However, how metabolic cues are integrated in the life cycle of LDs is unclear. This treatment triggers rapid consumption of LDs in WT cells but not in the Student . Nat Rev Mol Cell Bio doi: 10.1038/s41580-018-0085-z (2018). https://doi.org/10.1021/bi1013003 All data were acquired with Xcalibur software (v2.2; Thermo Fisher Scientific).Comprehensive lipidome analysis by shotgun lipidomics on a hybrid quadrupole-orbitrap-linear ion trap mass spectrometerSterol transporters at membrane contact sites regulate TORC1 and TORC2 signalingA sterol-enriched vacuolar microdomain mediates stationary phase lipophagy in budding yeastFluorescence microscopy was performed at room temperature (∼23°C) in a Cell Observer high speed microscope (ZEISS) equipped with a CMOS camera (ORCA-Flash 4.0; Hamamatsu Photonics) controlled by Slidebook 6.0 software (3i).

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